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Essential for precise deacetylase activity in analytical and biomanufacturing workflows, reliable diacetinase performance is critical to obtaining reproducible experimental results. Before switching from native-source enzymes, researchers and IVD developers must address three core questions regarding activity, contamination, and lot-to-lot reliability. Active Recombinant Diacetinase (Cat#: D401-31H), expressed in E. coli, delivers verified performance against each metric.
1. Functional Parity: Recombinant vs. Native Activity
A side-by-side comparison in a released acetate assay confirms that Recombinant Diacetinase matches the activity of native-source enzymes, ensuring no functional trade-offs when transitioning sources.
- Unit Definition: One unit of activity is defined as the amount of enzyme that will catalyze the release of 1.0 micromole of acetate per minute at 37°C.
- Key Benefits: Confirms equivalent functional performance while reducing supply-chain risks and supporting lot-to-lot consistency.
2. Verified Low Contamination
To minimize off-target enzymatic interference and deliver cleaner assay signals in downstream applications, Recombinant Diacetinase undergoes specific contamination testing:
| Contaminating Enzymes | Specification | Result |
| Cholesterol Esterase | ≤1.00% | PASS |
| Glucose Oxidase | ≤0.0002 U/mg | PASS |
3. Defined Quality & Activity Specifications
Manufactured to strict, lot-by-lot quality specifications, Recombinant Diacetinase supports regulatory-grade reproducibility and reduces qualification burdens for IVD client workflows:
| Test Items | Specification | Verified Result |
| Activity | ≥0.2 U/mg-liquid | 0.4 U/mg-liquid |
| Purity | >99% (by densitometry) | >99% |
4. Product Overview & Key Advantages
- Product Details: Active Recombinant Diacetinase (Cat#: D401-31H), expressed in E. coli cells, supplied as a colourless, frozen liquid.
- Seamless Transition: Matches native-source enzyme activity performance.
- Cleaner Signals: Low cholesterol esterase and glucose oxidase contamination ensures specific, highly reproducible readouts.
- Reliable Sourcing: E. coli recombinant production eliminates dependency on native extraction and mitigates source variability.
- Documentation: Activity, purity, and concentration are spec-verified lot-by-lot for regulatory readiness.
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